{"product_id":"biolegend-699003","title":"Biolegend, 699003, PE anti-NTAL (LAT2) Antibody, 25tests","description":"\u003cp\u003eNon-T cell activation linker (NTAL) functions as transmembrane adaptor scaffold that links engagement of antigen receptor binding to downstream effector molecules, playing an essential role in maturation, activation, and differentiation in various cell types. It is important for BCR (B-cell antigen receptor) and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-signaling in B-cells and myeloid cells, respectively, and plays a critical role in FCER1 (high affinity immunoglobulin epsilon receptor) signaling in mast cells.  NTAL is encoded by the LAT2 gene, which is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial human NTAL recombinant protein (27-243 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This antibody does not cross-react with mouse NTAL (in-house tested). Daudi cells were selected for antibody testing due to the cell line’s low expression level of the mRNA encoding NTAL (source: Human Protein Atlas).\u003cbr\u003e\nRRID: AB_2721643 (BioLegend Cat. No. 699004)\u003cbr\u003e\nStructure: 243 amino acids with a predicted molecular weight of 26.5 kD, palmitoylation moiety permits localization to lipid rafts or tetraspanin-enriched microdomains.\u003cbr\u003e\nDistribution: Plasma membrane\u003cbr\u003e\nFunction: NTAL is extensively phosphorylated on multiple tyrosine residues following activation, enabling the protein to serve as a scaffold for multiple signaling complexes in various cell contexts. May also be post-translationally regulated by Caspase-3-mediated proteolytic cleavage in human B cells and monocytes.\u003cbr\u003e\nInteraction: Phospholipase C gamma 1 and 2. When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1, and CBL.\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Arbulo-Echevarria MM, et al. 2016. J. Leukoc Biol. 100:351. 2. Stepanek O, et al. 2014. Cell Signal. 5:895. 3. Orr Sj, et al. 2013. Plos Pathog. 9: e1003357. 4. Halova I, et al. 2013. J. Biol. Chem. 288:9801. 5. Duque-Alfonso J, et al. 2011. Oncogene. 30:3062\u003cbr\u003e\nGene ID: 7462\u003cbr\u003e\nUniProt: View information about NTAL on UniProt.org\u003cbr\u003e\nClone: W15102A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NTAL, LAT2, WBS15, LAB\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866298732713,"sku":"699003","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-699003","provider":"Iright","version":"1.0","type":"link"}