{"product_id":"biolegend-710254","title":"Biolegend, 710254, Recombinant Human BMP-3b\/GDF-10 (carrier-free), 25μg","description":"\u003cp\u003eBone Morphogenetic Protein 3b (BMP-3b), also known as Growth Differentiation Factor 10 (GDF-10), is a member of the Transforming Growth Factor-beta (TGF-β) superfamily. Although both BMP-3 and BMP-3b belong to the BMP family, they exhibit distinct structural characteristics. BMP-3b shares approximately 82% amino acid sequence identity with BMP-3 in the mature region and 37% in the pro-domain. Like other BMP family members, BMP-3 is synthesized as an inactive propeptide precursor, which dimerizes and is subsequently processed into its mature form. Functionally, BMP-3 acts as an antagonist to BMP-2-induced osteogenic signaling, inhibiting the differentiation of osteoprogenitor cells into osteoblasts. BMP-3 antagonizes the BMP type II receptor ActRIIB and serves as a negative regulator of bone density. BMP-3 expression is observed in osteoblasts at erosion sites during the late stages of inflammatory arthritis and has been associated with colorectal cancer and gastric carcinoma. Overexpression of BMP-3 during skeletal development modifies endochondral bone formation resulting in spontaneous rib fractures. BMP-3b is expressed in various tissues, including the brain, skeletal tissue, and adipose tissue. It plays key roles in neurodevelopment, bone formation, adipogenesis, metabolic regulation, tissue homeostasis, and oncogenesis. BMP-3b treatment has been shown to promote neuronal growth and improve functional recovery in a mouse model of stroke. Additionally, in a neonatal mouse model of hypoxia-ischemia-induced encephalopathy, BMP-3b administration enhanced survival outcomes. BMP-3b also acts as a negative regulator of osteoblast differentiation.\u003cbr\u003e\n25μg\u003cbr\u003e\nSource: Recombinant human BMP-3b\/GDP-10, amino acid Lys368-Arg478 (Accession No. P55107) without any tag was expressed in E.coli.\u003cbr\u003e\nMolecular Mass: The 112 amino acid recombinant protein has a predicted molecular mass of approximately 12.7 kD. The DTT-reduced protein and the non-reduced protein migrate at approximately 10 - 15 and 20 - 30 kD by SDS-PAGE, respectively. The N-terminal amino acid is Met.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in 0.1% TFA, 10% acetonitrile\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method.\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Recombinant human BMPR1B\/ALK-6 binds to immobilized recombinant human BMP-3b\/GDP-10 in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.5 µg\/mL.\u003cbr\u003e\nApplication: Bioassay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: Dimer\u003cbr\u003e\nDistribution: Brain, skeletal tissue, and adipose tissue\u003cbr\u003e\nFunction: Neurodevelopment, bone formation, and adipogenesis\u003cbr\u003e\nInteraction: Osteoblasts, granulosa cells of growing follicles, mammary stem cells, neurons, mammary gland, muscle cells, fibroblasts\u003cbr\u003e\nLigand\/Receptor: ALK-4\/ACVR1B, ALK-6\/BMPR1B, ALK-3\/BMPR1A, ACVR2A, ACVR2B, ALK-2\/ACVR1\u003cbr\u003e\nBioactivity: Measured by its ability to bind ALK-6\/BMPR1B\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Fibroblasts, Macrophages, Neurons, T cells\u003cbr\u003e\nBiology Area: Cardiovascular Biology, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nAntigen References: He F, et al. 2022. Carcinogenesis. 43(2):94. Zhang Y, et al. 2025. Adv Sci. 12(19):e2500616. Upadhyay G, et al. 2011. Proc Natl Acad Sci U S A. 108(19):7820. Uezumi A, et al. 2021. J Clin Invest. 131(1): e139617. Martí-Pàmies Í, et al. 2023. Circ Res. 133(4): 353. Zhao R, et al. 1999. Dev Biol. 212 (1):68. Suzuki A, et al. 2025. Am J Respir Cell Mol Biol. 67(4):446. Li S, et al. 2015. Nat Neurosci. 18(12):1737. Ogawa Y, et al. 2018. Front Neurol. 9:397. Daluiski A, et al. 2001. Nat Genet. 27:84. Hino J, et al. 2004. Front Biosci. 9:1520.\u003cbr\u003e\nGene ID: 2662\u003cbr\u003e\nUniProt: View information about GDF-10\/hBMP-3b on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bone morphogenetic protein 3B, BMP3b, BMP-3b, growth differentiation factor 10, GDF-10, GDF10\u003cbr\u003e\nQ: Why choose BioLegend recombinant proteins?\u003cbr\u003e\nA: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.\u003cbr\u003e\n     • Greater than 95% Purity or higher, tested on every lot of product.\u003cbr\u003e\n     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.\u003cbr\u003e\n     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.\u003cbr\u003e\n     • Bulk and customization available. Contact us.\u003cbr\u003e\n     • Learn more about our Recombinant Proteins.\u003cbr\u003e\nQ: How does the activity of your recombinant proteins compare to competitors?\u003cbr\u003e\nA: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!\u003cbr\u003e\nQ: What is the specific activity or ED50 of my recombinant protein?\u003cbr\u003e\nA: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells\/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.\u003cbr\u003e\nQ: Have your recombinants been tested for stability?\u003cbr\u003e\nA: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.\u003cbr\u003e\nQ: Does specific activity of a recombinant protein vary between lots?\u003cbr\u003e\nA: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.\u003cbr\u003e\nQ: How do you convert activity as an ED50 in ng\/ml to a specific activity in Units\/mg?\u003cbr\u003e\nA: Use formula Specific activity (Units\/mg) = 10^6\/ ED50 (ng\/mL)\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864224452777,"sku":"710254","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-710254","provider":"Iright","version":"1.0","type":"link"}