{"product_id":"biolegend-712054","title":"Biolegend, 712054, Biotinylated Recombinant Human Cadherin-17 (carrier-free), 25μg","description":"\u003cp\u003eCadherin-17 (CDH-17), also known as liver-intestine (LI) cadherin, is a transmembrane glycoprotein and a member of the cadherin superfamily of calcium-dependent cell adhesion molecules. It contains seven extracellular cadherin domains and is primarily expressed in the gastrointestinal tract, including the colon, small intestine, and liver. CDH-17 plays a crucial role in cell-cell adhesion within the intestinal epithelium and pancreatic ducts. Overexpression of CDH-17 has been observed in various cancers, including hepatocellular carcinoma and gastric cancer. Studies have shown that knocking down CDH-17 in gastric and hepatocellular carcinoma cell lines can inhibit cell proliferation, migration, invasion, adhesion, and colony formation in vitro . In vivo , CDH-17 knockout has been linked to reduced tumor growth in xenograft models and increased apoptotic tumor cell death through caspase-3 activation. These findings highlight CDH-17 as a key player in tumorigenesis and a potential diagnostic marker for gastrointestinal and pancreatic cancers.\u003cbr\u003e\n25μg\u003cbr\u003e\nSource: Recombinant human Cadherin-17, amino acid Gln23-Met787 (Accession No. Q12864) was expressed in 293E cells. The C-terminal includes Avi-6His.\u003cbr\u003e\nMolecular Mass: The 801 amino acid recombinant protein has a predicted molecular mass of approximately 91 kD. The DTT-reduced and non-reduced proteins migrates at approximately 80 - 115 kD by SDS-PAGE. The predicted N-terminall amino acid is Gln.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in PBS, pH 7.2\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method.\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Biotinylated recombinant human CDH17 binds to immobilized recombinant human Galectin-9 in a dose-dependent manner. The ED50 for this effect is 1 - 6 µg\/mL\u003cbr\u003e\nApplication: Bioassay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: Monomer\u003cbr\u003e\nDistribution: Intestinal cells, B cells in embryonic development and fetal liver, memory B cells, cancer cells, epithelial cells in small intestine, colon, and pancreatic ducts\u003cbr\u003e\nFunction: Cell- cell adhesion, tissue morphogenesis, signal transduction\u003cbr\u003e\nInteraction: CDH1, p120-catenin\u003cbr\u003e\nLigand\/Receptor: α2β1 integrin\u003cbr\u003e\nBioactivity: Measured by its ability to bind biotinylated recombinant human Galectin-9\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Epithelial cells, Erythrocytes\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adaptor Proteins\u003cbr\u003e\nAntigen References: Dantzig AH, et al. 1994. Science. 264:430-3. Liu LX. et al. 2009. Hepatology. 50:1453-63. Ding ZB, et al. 2009. Cancer. 115:4753-65. Bartolmäs T, et al. 2012. Cell Mol Life Sci. 69:3851-62. Feng Z. et al. 2022. Nat Cancer. 3:581. Liu X. et al. 2019. Cancer Lett. 454:204-214. Funakoshi S, et al. 2015. PLoS One.10:e0117566.\u003cbr\u003e\nGene ID: 1015\u003cbr\u003e\nUniProt: View information about Cadherin-17 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LI cadherin (liver-intestine), Cadherin 17, Cadherin17,Cadherin-17, CDH16, CDH17, FLJ26931, HPT-1 cadherin, HPT1,HPT-1, human intestinal peptide-associated transporter HPT-1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864224321705,"sku":"712054","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-712054","provider":"Iright","version":"1.0","type":"link"}