{"product_id":"biolegend-715854","title":"Biolegend, 715854, Biotinylated Recombinant Human SLAMF4 (CD244\/2B4)-Fc Chimera (carrier-free), 25μg","description":"\u003cp\u003eSLAMF4, also known as 2B4 and CD244, is a member of the CD2 subfamily. It is expressed in NK cells, monocytes, basophils, TCR γ\/δ T cells, and a subset of CD8+ T cells. Engagement of SLAMF4 by antibodies or its natural ligand, CD48, leads to tyrosine phosphorylation of SLAMF4, cell-mediated cytotoxicity, and IFN-γ secretion by NK cells. Except for CD48 ligation, SLAMF4-mediated non-MHC-restricted cell cytotoxicity is also dependent on other NCRs, such as NKp46 (NCR1\/CD335). However, engagement of SLAMF4 with inhibitory receptors, such as KIR2DL1 or NKG2 (CD94), blocks SLAMF4-mediated cell cytotoxicity and cytokine production. The cytoplasmic region of SLAMF4 contains several immunoreceptor tyrosine-based switch motifs (ITSMs), which serve as activating and inhibitory regulatory elements for SLAMF4-mediated signaling. Its ITSMs can not only bind to SAP and Src kinase Fyn but also recruit the inhibitory kinase, Csk, and phosphatase SHP-1, SHP-2, and SHIP, leading to positive and negative signaling on cells. The activating and inhibitory functions are dependent on the density of surface expression of SLAMF4, degree of ligation, and the level of the adaptor molecule SAP expression. In XLP (X-linked lymphoproliferative disease) patients, SLAMF4 engagement cannot activate NK cells due to impairment of SAP\/SH2D1A association. It has been demonstrated that SLAMF4 interacts with CD48 and functions as a costimulatory molecule, playing a critical role in cell proliferation and MHC-restricted cytotoxicity in CD8+ T cells. SLAMF4 expression also correlates with T-cell differentiation. CD8+ effector αβ T cells predominantly express surface SLAMF4 with high levels of granzyme B and perforin expression, and rapid production of IFN-γ.\u003cbr\u003e\n25μg\u003cbr\u003e\nSource: Human SLAMF4 (CD244\/2B4), amino acid (Cys22-Arg221) (Accession# NP_057466.1), with a linker, a C-terminal Human IgG (Pro100-Lys330), and an Avi-tag, was expressed in CHO cells. The Recombinant Human CD244-Avi tag was site-specifically biotinylated by the enzyme BirA.\u003cbr\u003e\nMolecular Mass: The 450 amino acid recombinant protein has a predicted molecular mass of approximately 50.4 kD. The DTT-reduced and non-reduced proteins migrate at approximately 65 -85 kD and 110 – 145 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Cys.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie-stained SDS-PAGE\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in PBS, 5% Sucrose, pH 7.4\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg cytokine as determined by the LAL method\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: When Recombinant Human SLAMF2 (Cat. No. 788706) is immobilized at 5 µg\/mL, Biotinylated Recombinant Human SLAMF4 (CD244\/2B4) binds in a dose-dependent manner with an ED50 range of 15 – 90 ng\/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding.\u003cbr\u003e\nApplication: Biossay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: Dimer\u003cbr\u003e\nDistribution: NK cells, monocytes and basophils, TCR γ\/δ T cells, and a subset of CD8+ T cells\u003cbr\u003e\nFunction: Engagement of SLAMF4 with CD48 stimulates cell-mediated cytotoxicity and cytokine secretion\u003cbr\u003e\nInteraction: NK cells, subsets of T cells\u003cbr\u003e\nLigand\/Receptor: SLAMF2\/CD48\u003cbr\u003e\nBioactivity: The bioactivity was measured by immobilizing the Recombinant Human SLAMF2 binding with Biotinylated Recombinant Human SLAMF4 (CD244\/2B4) in a dose-dependent manner using the functional ELISA.\u003cbr\u003e\nCell Type: Basophils, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Soluble Receptors\u003cbr\u003e\nAntigen References: Parolini S, et al. 2000. J Exp Med. 192:337-46. Watzl C, et al. 2000. J Immunol. 165:3545-8. Brown MH, et al. 1998. J Exp Med. 188:2083-90. Watzl C, et al. 2000. J Immunol. 165:3545-8. Eissmann P, et al. 2005. Blood. 105:4722-9. Latchman Y, et al. 1998. J Immunol. 161:5809-12. Speiser DE, et al. 2001. J Immunol. 167:6165-70. Lee KM, et al. 2003. J Immunol. 170:4881-5. Kambayashi T, et al. 2001. J Immunol. 167:6706-10. Tangye SG, et al. 1999. J Immunol. 162:6981-5. Nakajima H, et al. 1999. Eur J Immunol. 29:1676-83. Schuhmachers G, et al. 1995. Eur J Immunol. 25:1117-20 Sivori S, et al. 2000. Eur J Immunol. 30:787-93.\u003cbr\u003e\nGene ID: 51744\u003cbr\u003e\nUniProt: View information about SLAMF4 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SLAMF4, SLAM family member 4, 2B4, Natural killer cell receptor2B4, NK cell activation-inducing ligand, NAIL, NK cell type Ireceptor protein 2B4, NKR2B4, h2B4, signaling lymphocyticactivation molecule 4, CD244\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864268525737,"sku":"715854","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-715854","provider":"Iright","version":"1.0","type":"link"}