{"product_id":"biolegend-717356","title":"Biolegend, 717356, Recombinant Human CD97-Fc Chimera (carrier-free), 100μg","description":"\u003cp\u003eCD97 is a GPCR family member with a long ECD joined to the seven transmembrane domains by a GPCR proteolytic site (GPS). The ECD possesses three to five EGF domains in the NH2 terminus, two contain a calcium binding site. The EGF domains bind to CD55 through its N-terminal short consensus repeat (SCR).  CD97 is an early activation marker in T cells and the interaction with human complement regulator, decay-accelerating factor (DAF, CD55) shows a strong costimulatory signal to human T cells. The interaction of CD97 and CD55 is species-restrictive (human CD97 does not bind mouse CD55). CD97 interacts with α5β1 and αvβ3 integrins and stimulates angiogenesis in endothelial cells. In addition, CD97 interacts with CD90 in activated endothelial cells. CD97 is widely expressed in different normal and in cancer cells. CD97 and lysophosphatidic acid receptor 1 (LPAR1) heterodimerize and signal through G α12\/13 to increase RHO-GTP levels to promote tumor invasion in prostate and thyroid cancers. High expression of CD97 has been found in pancreatic, gastric, and esophageal carcinomas. Worse clinical stage has been associated with high expression of CD97 in colorectal carcinoma. CD97 and GPCR kinase 6 (GRK6) synergize to promote metastasis of hepatocellular carcinoma (HCC) through the induction downstream of MMP2\/MMP9.\u003cbr\u003e\n100μg\u003cbr\u003e\nSource: Recombinant human CD97-Fc Chimera, amino acid sequence containing Gln21- Gln398 (Accession No. NM_001784.4) was expressed in 293E cells. The carboxy-terminus contains SR-IEGRMD-hIgG1 (Pro100-Lys330)-Fc tag.\u003cbr\u003e\nMolecular Mass: The 617 amino acid recombinant protein has a predicted molecular mass of approximately 68 kD. The DTT-reduced and non-reduced proteins migrate at approximately 80 - 90 kD and 180 - 210 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Gln.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.2 µm filtered protein solution is in PBS, pH 7.0\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg cytokine as determined by the LAL method.\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Recombinant human CD97-Fc Chimera supports the adhesion of human red blood cells in a dose dependent manner. The ED50 for this effect is 0.05 – 0.4 µg\/mL.\u003cbr\u003e\nApplication: Bioassay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: Dimer\u003cbr\u003e\nDistribution: T cells, cancer cells, lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle\u003cbr\u003e\nFunction: Cell adhesion, migration, regulation of intercellular junctions\u003cbr\u003e\nInteraction: T cells, red blood cells\u003cbr\u003e\nLigand\/Receptor: CD55, α5β1 and αvβ3 integrins, CD90\u003cbr\u003e\nBioactivity: Measured by its ability to support the adhesion of human red blood cells.\u003cbr\u003e\nCell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Fc Receptors, GPCR, Soluble Receptors\u003cbr\u003e\nAntigen References: Hamann J, et al. 1996. J. Exp. Med. 184:1185. Qian YM, et al. 1999. Immunology. 98:303. Steinert M, et al. 2002. Am J Pathol. 161:1657. Wang T, et al. 2004. Blood. 105:2836. Spendlove I and Sutavani R. 2010. Adv Exp Med Biol. 706:138. Wandel E, et al. 2012. J Immunol. 188:1442. Ward Y, et al. 2013. Oncogene. 32:2726. Lang Li-Yung, et al. 2017. Acta Pharmacol Sin. 38:56. Yin Y, et al. 2018. Hepatology. 68:1865. Qiuming S, et al, 2022. World J. Surg Oncl. 20:382.\u003cbr\u003e\nGene ID: 976\u003cbr\u003e\nUniProt: View information about CD97 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TM7LN1, ADGRE5\u003cbr\u003e\nQ: Why choose BioLegend recombinant proteins?\u003cbr\u003e\nA: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.\u003cbr\u003e\n     • Greater than 95% Purity or higher, tested on every lot of product.\u003cbr\u003e\n     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.\u003cbr\u003e\n     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.\u003cbr\u003e\n     • Bulk and customization available. Contact us.\u003cbr\u003e\n     • Learn more about our Recombinant Proteins.\u003cbr\u003e\nQ: How does the activity of your recombinant proteins compare to competitors?\u003cbr\u003e\nA: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!\u003cbr\u003e\nQ: What is the specific activity or ED50 of my recombinant protein?\u003cbr\u003e\nA: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells\/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.\u003cbr\u003e\nQ: Have your recombinants been tested for stability?\u003cbr\u003e\nA: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.\u003cbr\u003e\nQ: Does specific activity of a recombinant protein vary between lots?\u003cbr\u003e\nA: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.\u003cbr\u003e\nQ: How do you convert activity as an ED50 in ng\/ml to a specific activity in Units\/mg?\u003cbr\u003e\nA: Use formula Specific activity (Units\/mg) = 10^6\/ ED50 (ng\/mL)\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864208330921,"sku":"717356","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-717356","provider":"Iright","version":"1.0","type":"link"}