{"product_id":"biolegend-773301","title":"Biolegend, 773301, Prime-Step™ Prestained Broad Range Protein Ladder, 50microl","description":"\u003cp\u003eThe Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. It has 7 blue-stained bands and 3 reference bands (2 orange bands at 30 kDa and 270 kDa and 1 green band at 52 kDa) when separated on Tris-glycine gel. The Prime-Step™ Prestained Broad Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, confirming Western blot transfer quality on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot.   Key Product Features\u003cbr\u003e\n50microl\u003cbr\u003e\nFormulation: Sample loading buffer (62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol).\u003cbr\u003e\nPreparation: Chromophore conjugated recombinant protein mixture supplied in sample loading buffer.\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store at -20°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: • 3-5 μl per loading for clear visualization during electrophoresis on 15-well mini-gel or 20-well midi-gel, respectively. • 3-5 μl per well for general Western transferring. The ladder is supplied in the sample loading buffer and is ready to use.\u003cbr\u003e\nApplication Notes: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards.\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: protein ladder, protein marker, pre-stained protein ladder, pre-stained protein marker, protein molecular weight marker\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866501959849,"sku":"773301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-773301","provider":"Iright","version":"1.0","type":"link"}