{"product_id":"biolegend-801601","title":"Biolegend, 801601, Purified anti-Neurofilament H (NF-H), Phosphorylated Antibody (Previously Covance catalog# SMI 31P), 100microl","description":"\u003cp\u003eNeurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution + Thimerosal.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.\u003cbr\u003e\nApplication: IHC-P - Quality tested WB, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg\/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg\/ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg\/ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunohistochemistry2,4, and immunocytochemistry4. SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Barry D, et al. 2012. J. Neurosci. 32:6209 (WB) PubMed Choi Y, et al. 2008. Genes Dev. 22:2485. (IHC) PubMed Sepulveda B, et al. 2013. PLoS ONE. 8(e61986. (ICC) PubMed McLean NA, et. al. 2014. PLoS One 9:e110174. (IHC) PubMed\u003cbr\u003e\nProduct Citations: Takahashi M, et al. 2015. J Neurol Neurosurg Psychiatry. 86: 939-944. PubMed Johari M, et al. 2021. Acta Neuropathologica. . PubMed Fleuriet J, et al. 2020. Sci Rep. 10:11927. PubMed Seiler S, et al. 2023. Sci Rep. 13:2883. PubMed D'mello V, et al. 2023. Neurotrauma Rep. 4:236. PubMed So HK, et al. 2023. Research (Wash D C). 6:0158. PubMed Frondelli MJ, et al. 2022. J Neurosci Res. 100:578. PubMed Powers RM, et al. 2022. Mol Biol Cell. 33:ar64. PubMed Cunningham ME 2022. Experimental Neurology. 355:114127. PubMed Won D, et al. 2022. Sci Adv. 8:eabo3209. PubMed Hu T, et al. 2022. Cell Biosci. 12:196. PubMed Pellegatta M, et al. 2022. J Neurosci. 42:2433. PubMed Zhu PP, et al. 2022. Hum Mol Genet. 31:2779. PubMed von Saucken VE, et al. 2020. Neurobiol Dis. 145:105072. PubMed Lavoie–Cardinal F, et al. 2020. Sci Rep. 10:11960. PubMed McGonigal R, et al. 2021. Journal of Neurochemistry. . PubMed Alada-Morais S, et al. 2021. Cerebral Cortex. :. PubMed Miguel–Hidalgo JJ, et al. 2019. Neuroscience. 411:255. PubMed Mahajan KR, et al. 2020. Ann Neurol. 88:81. PubMed Wang S et al. 2019. Front Cell Neurosci. 0.766666667 . PubMed Cui X, et al. 2016. Stroke. 47: 214 - 220. PubMed Berghoff S, et al. 2017. Nat Commun. 8:14241. PubMed Andrew RJ, et al. 2017. Proc Natl Acad Sci U S A. 114:E9665. PubMed Özen I, et al. 2022. Acta Neuropathol Commun. 10:129. PubMed Barry D, et al. 2012. J Neurosci. 32:6209-6219. PubMed Cui C, et al. 2016. Stem Cells Trans Med. 5(12):1656-1667. PubMed Wang W, et al. 2022. Dose Response. 20:15593258221112959. PubMed McGonigal R, et al. 2022. J Clin Invest. :. PubMed Choi Y, et al. 2008. Genes Dev. 22:2485-2495. PubMed Manangeeswaran M, et al. 2022. Front Immunol. 13:919815. PubMed Maggiore JC, et al. 2020. J Tissue Eng Regen Med. 14:1892. PubMed Xu X, et al. 2022. Sci Rep. 12:14690. PubMed Stumpf SK, et al. 2019. Acta Neuropathol. 138:147. PubMed Scaramuzzino C, et al. 2022. Sci Adv. 8:eabj8812. PubMed McGonigal R et al. 2018. The Journal of Neuroscience. 39(1):63-77 . PubMed Chaves RS, et al. 2021. J Neurosci. 41:10034. PubMed Maire C, et al. 2014. Stem Cells. 32:313-326. PubMed Redondo J, et al. 2015. Brain Pathol. 25:692-700. PubMed Generous AR, et al. 2019. J Cell Sci. 132:jcs235507. PubMed Cuadrado E et al. 2019. Cell reports. 26(7):1718-1726 . PubMed Halabi EA, et al. 2019. Nat Commun. 10:1232. PubMed McLean N, et al. 2014. PLoS One. 9:e110174. PubMed Riku Y, et al. 2016. J Neuropathol Exp Neurol. 75: 801 - 811. PubMed Yu G, et al. 2018. Neuroreport. 28:29. PubMed Kiryu-Seo S, et al. 2010. J Neurosci. 30:6658-6666. PubMed Kubo A, et al. 2019. J Comp Neurol. 527:985. PubMed Bishop R, et al. 2022. Sci Rep. 12:143. PubMed Doroshenko ER, et al. 2021. Front Immunol. 570425:12. PubMed Cooper ML, et al. 2020. Proc Natl Acad Sci U S A. 117:18810. PubMed Li T, et al. 2016. Nat Commun. 7:12082. PubMed Schultz V, et al. 2021. Glia. 69:2023. PubMed\u003cbr\u003e\nRRID: AB_2715851 (BioLegend Cat. No. 801602) AB_2564641 (BioLegend Cat. No. 801601)\u003cbr\u003e\nStructure: Neurofilament H has an apparent molecular mass of 200-220 kD.\u003cbr\u003e\nDistribution: Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate. Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.\u003cbr\u003e\nFunction: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.\u003cbr\u003e\nInteraction: Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments, Phospho-Proteins\u003cbr\u003e\nAntigen References: 1. Petzold A. 2005. J. Neurol. Sci. 233 (1-2):183. PubMed\u003cbr\u003e\nGene ID: 4744\u003cbr\u003e\nUniProt: View information about Neurofilament H on UniProt.org\u003cbr\u003e\nClone: SMI 31\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein\u003cbr\u003e\nPreviously: Covance Catalog# SMI 31P\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866294997161,"sku":"801601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-801601","provider":"Iright","version":"1.0","type":"link"}