{"product_id":"biolegend-805504","title":"Biolegend, 805504, Biotin anti-β-Amyloid, 1-42 (Previously Covance catalog# SIG-39144), 200microl","description":"\u003cp\u003eAmyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as \"seeds\") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.\u003cbr\u003e\n200microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution (no preservatives or carrier proteins).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Do not store antibody diluted below 50 µg\/mL in the absence of protein (i.e.: add 2% of bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.\u003cbr\u003e\nApplication: IHC-P - Quality tested Direct ELISA - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.25 – 1.0 µg\/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.01 – 1.0 µg\/ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 42nd amino acid.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Helwig M, et al. 2013. J. Biol. Chem. 288:1114. (IHC-P) Takami M, et al.2009. J. Neurosci.; 29: 13042. (IHC-P) Cenini G, et al. 2012. Biochim Biophys Acta. 1822(2):130. (ELISA) PubMed Sundaram RK, et al. 2012. Int. J. Pept. Res. Ther. 18(2):99. (ELISA) PubMed Savage MJ, et al. 2014. J. Neurosci. 8:2884. (ELISA) PubMed Mastrangelo MA, Bowers WJ. 2008. BMC Neurosci. 9:81. (IHC-F) PubMed\u003cbr\u003e\nProduct Citations: Cenini G, et al. 2012. Biochim Biophys Acta. 1822:130-138. PubMed Zhu Q, et al. 2022. Med Phys. 49:1357. PubMed Townsend M, et al. 2010. J Pharmacol Exp Ther. 333:110:119. PubMed Gutknecht MF, et al. 2022. Cell Rep Methods. 2:100214. PubMed Wu Y, et al. 2019. Nat Commun. 10:58. PubMed Sundaram R, et al. 2012. Int J Pept Res Ther. 18:99-106. PubMed\u003cbr\u003e\nRRID: AB_2564688 (BioLegend Cat. No. 805504)\u003cbr\u003e\nStructure: Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.\u003cbr\u003e\nDistribution: Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle. Distribution: cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.\u003cbr\u003e\nFunction: The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nMolecular Family: APP\/β-Amyloid\u003cbr\u003e\nAntigen References: Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1 Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83. Puig KL, et al. 2012. Exp. Gerontol. 48(7): 608. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595. Walsh DM, et al. 2007. J. Neurochem. 101(5):1172.\u003cbr\u003e\nGene ID: 351\u003cbr\u003e\nUniProt: View information about beta-Amyloid 1-42 on UniProt.org\u003cbr\u003e\nClone: 12F4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein\u003cbr\u003e\nPreviously: Signet Catalog# 9144-02Signet Catalog# 9144-05Signet Catalog# 9144-10Covance Catalog# SIG-39144\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860796461225,"sku":"805504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-805504","provider":"Iright","version":"1.0","type":"link"}