{"product_id":"biolegend-824201","title":"Biolegend, 824201, Purified anti-Histone H3 Monomethyl (Lys9) Antibody (Previously Covance catalog# MMS-5084), 100microl","description":"\u003cp\u003eChromosomal DNA wraps around histone proteins located in the nucleus of the cell. Histone H3 is one of the five main histone proteins. Featuring a main globular domain and a long N-terminal tail, H3 forms nucleosomes with the 'beads on a string' structure. Histone proteins are highly post-translationally modified and Histone H3 is the most extensively modified. The term \"Histone H3\" does not distinguish between sequence variants or modification state - including three sequence variants H3.1, H3.2, and H3.3. Histone H3 is important in the field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Histone H3 lysine 9 (H3K9) is a major site of chromatin regulation. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids as well as phosphorylation events. Di- and Tri-methylation of Lysine 9 are associated with repression and heterochromatin, while mono-methylation of K4 is associated with active genes and has been associated with gene silencing and the formation of relatively inactive regions of DNA known as heterochromatin. When H3K9 is acetylated, its neutral charge cannot bind to DNA, resulting in greater access of transcriptional enzymes to DNA. When H3K9 is unmodified, less transcription occurs. The greater the methylation on H3K9, the greater the transcriptional repression.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nReported Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: This monoclonal antibody was raised against a synthetic peptide from human histone H3, mono-methylated at Lysine-9.\u003cbr\u003e\nFormulation: Phosphate-buffered solution.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.\u003cbr\u003e\nApplication: WB, IHC\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100 - 1000* • IHC: 1:100 Tissue: Frozen sections Pretreatment: None Primary Incubation: 24 hours at 4°C\u003cbr\u003e\nApplication Notes: This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC). * Expected MW: 17 kD WB Positive control: HeLa cell lysate IHC Positive control tissue: Normal mouse brain This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).\u003cbr\u003e\nProduct Citations: Wimmers F, et al. 2021. Cell. 184:3915. PubMed\u003cbr\u003e\nRRID: AB_2564878 (BioLegend Cat. No. 824201)\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Rice JC1 and Allis CD. 2001. Curr. Opin. Cell. Biol. 13:263.\u003cbr\u003e\nGene ID: 8350\u003cbr\u003e\nUniProt: View information about Histone H3 on UniProt.org\u003cbr\u003e\nClone: 7E7.H12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Histone H3.1, histone H3\/a, histone 1, H3\/a, H3 histone family, member A\u003cbr\u003e\nPreviously: Covance Catalog# MMS-5084\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862488404137,"sku":"824201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-824201","provider":"Iright","version":"1.0","type":"link"}