{"product_id":"biolegend-835602","title":"Biolegend, 835602, Anti-Neurofilament H \u0026 M (NF-H\/NF-M), Hypophosphorylated Antibody (Previously Covance catalog# SMI-35R), 500microl","description":"\u003cp\u003eNeurofilaments (NFs) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest neurofilament (NF-L) runs at 68-70 kD. The medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).\u003cbr\u003e\n500microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nPreparation: Ascites\u003cbr\u003e\nConcentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg\/ml.\u003cbr\u003e\nStorage \u0026amp; Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.\u003cbr\u003e\nApplication: IHC-P - Quality testedWB - VerifiedIHC-F, ELISA - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1:1000 - 1:2000 µg\/ml is suggested. For Western blotting, the suggested use of this reagent is 1:1000 - 1:5000 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats of this clone): include ELISA Capture1-3, immunohistochemical staining on frozen tissue sections, immunofluorescence staining, and spatial biology (IBEX)6,7. Clone SMI 35 reacts with highly phosphorylated neurofilaments, as well as with low degrees of phosphorylation. It primarily reacts with neurofilament H and with neurofilament M to a lesser extent. Notes: On two dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kD (pI 5.1) toward the non-phosphorylated position at 170 kD (pI 6.2).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Petzold A. 2013. J. Neuroimmunol. 262:(1-10). (ELISA) Lu CH, et al. 2012. PLoS One. 7:e40998. (ELISA) Steinacker P, et al. 2011. PLoS One. 8:e23600. (ELISA) Poltorak M, et al. 1993. J. Neurosci. 13:2217. (IHC-F) Petzold A, et al. 2011. Brain. 134:464. (WB) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Chung HL, et al. 2020. Neuron. 106(4):589-606. PubMed Lu C, et al. 2012. PLoS One. 7:e40998. PubMed Das S, et al. 2020. Commun Biol. 0.354166667. PubMed Neil S, et al. 2017. Brain Behav Immun. 10.1016\/j.bbi.2017.02.018. PubMed Steinacker P, et al. 2011. PLoS One. 6:e23600. PubMed\u003cbr\u003e\nRRID: AB_2565349 (BioLegend Cat. No. 835601) AB_2565350 (BioLegend Cat. No. 835602)\u003cbr\u003e\nStructure: The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.\u003cbr\u003e\nDistribution: Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate Cellular Distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion\u003cbr\u003e\nFunction: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.\u003cbr\u003e\nInteraction: Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Intermediate Filaments, Phospho-Proteins\u003cbr\u003e\nAntigen References: Petzold A. 2012. Mult Scler Int. 2012:217802. PubMed Gresle MM, et al. 2011. Mult Scler Int. 2011: 315406. PubMed\u003cbr\u003e\nGene ID: 4744\u003cbr\u003e\nUniProt: View information about Neurofilament HM NF-H NF-M Phospho on UniProt.org\u003cbr\u003e\nClone: SMI 35\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein\u003cbr\u003e\nPreviously: Covance Catalog# SMI-35R\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866296373417,"sku":"835602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-835602","provider":"Iright","version":"1.0","type":"link"}