{"product_id":"biolegend-848201","title":"Biolegend, 848201, Purified anti-Transthyretin, aggregated Antibody, 25μg","description":"\u003cp\u003eTransthyretin (TTR) is a carrier protein capable of transporting thyroid hormones in the plasma and cerebrospinal fluid, and retinol (vitamin A) in the plasma. TTR can form aberrant, insoluble amyloid fibrils that primarily deposit in a patient's heart, peripheral nerves, or both. The wild-type protein is associated with senile systemic amyloidosis (SSA) and is typically diagnosed in men over 70-years-old. Single or double mutations in TTR are primarily associated with familial amyloidotic polyneuropathy (FAP) or familial amyloid cardiopathy (FAC) which affects younger patients, often in their 30s. Clinical symptoms of TTR amyloidosis have historically been attributed to fibrils, but recent experimental evidence indicates that soluble TTR aggregates are also pathogenic.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: A 1:1 mixture of wild-type Transthyretin fibrils and soluble aggregates of Y78F TTR.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: Direct ELISA - Quality tested ELISA Capture, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. For Direct ELISA, the optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. For ELISA capture applications, a concentration of 2.0 µg\/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration of is 1.0 - 5.0 µg\/mL is recommmended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 2T5C9 recognizes a mid-region epitope on transthyretin aggregates.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Phay M, et al. 2014. Rejuvenation Res. 17(2):97. (Direct ELISA, Capture ELISA, IHC) PubMed\u003cbr\u003e\nRRID: AB_2632817 (BioLegend Cat. No. 848201) AB_2632818 (BioLegend Cat. No. 848202)\u003cbr\u003e\nStructure: Transthyretin (TTR) is a β-sheet rich homotetrameric protein that has a molecular mass of 55 kD. Each monomer consists of 127 amino acids.\u003cbr\u003e\nDistribution: Primarily extracellular in blood and cerebrospinal fluid.\u003cbr\u003e\nFunction: The normal function of TTR is to transport thyroxine and retinol-binding protein in the blood and cerebrospinal fluid.\u003cbr\u003e\nInteraction: Perlecan, receptor for advanced glycation end product (RAGE).\u003cbr\u003e\nLigand\/Receptor: Thyroxine, retinol-binding protein bound to retinol.\u003cbr\u003e\nCell Sources: Liver, choroid plexus epithelium, retina pigment epithelium.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nAntigen References: 1. Plante-Bordeneuve V, Said G. 2011. Lancet Neurol. 10:1086. PubMed 2. Pinney JH, et al. 2013. J. Am. Heart Assoc. 2:e000098. PubMed 3. Hawkins PN, et al. 2015. Ann Med. 47(8):625. PubMed\u003cbr\u003e\nGene ID: 7276\u003cbr\u003e\nUniProt: View information about Transthyretin on UniProt.org\u003cbr\u003e\nClone: 2T5C9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PALB, ATTR, Prealbumin, TBPA, TTR\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862332133545,"sku":"848201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-848201","provider":"Iright","version":"1.0","type":"link"}