{"product_id":"biolegend-851406","title":"Biolegend, 851406, Biotin anti-Tau, 359-373 Antibody, 100μg","description":"\u003cp\u003eTau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyperphosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant fragment of human 4R Tau protein (K18) encompassing the microtubule binding domains.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 5.0 µg\/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody cross-reacts with 3R and 4R human Tau isoforms, and recognizes endogenous human, mouse, and rat Tau in brain lysates by WB.\u003cbr\u003e\nRRID: AB_2801197 (BioLegend Cat. No. 851405) AB_2801198 (BioLegend Cat. No. 851406)\u003cbr\u003e\nStructure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.\u003cbr\u003e\nDistribution: Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle. Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.\u003cbr\u003e\nFunction: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.\u003cbr\u003e\nInteraction: Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine\/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation\u003cbr\u003e\nMolecular Family: Tau\u003cbr\u003e\nAntigen References: Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.\u003cbr\u003e\nGene ID: 4137\u003cbr\u003e\nUniProt: View information about Tau 359-373 on UniProt.org\u003cbr\u003e\nClone: A16097B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Microtubule associated protein tau\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864400253097,"sku":"851406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-851406","provider":"Iright","version":"1.0","type":"link"}