{"product_id":"biolegend-853501","title":"Biolegend, 853501, Purified anti-FBXO7 Antibody, 25μg","description":"\u003cp\u003eF-box only protein 7 (FBXO7) is involved in the ubiquitination process during protein degradation. FBXO7 is a subunit of the SCF (SKP1\/cullin‐1\/F‐box protein) complex. The scaffold proteins cullin‐1 and SKP1, the RING subunit Rbx1 together with the substrate‐recruiting subunit FBXO7 form a functional E3 ubiquitin ligase. FBXO7 is implicated in Parkinson’s disease. In addition to reduced proteasome activity, loss of FBXO7 in mice leads to Parkinsonism-like phenotype. Human FBXO7 is mainly localized to the nucleus, while the mutation observed in patients with parkinsonian-pyramidal syndrome leads to impaired nuclear localization of this protein.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Human FBXO7 recombinant protein (1-250 a.a.) expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This antibody is specific for human FBXO7 and does not cross-react with rat or mouse FBXO7, verified by western blot. The two bands observed in western blot potentially indicate the two isoforms of FBXO7.\u003cbr\u003e\nRRID: AB_2734650 (BioLegend Cat. No. 853501) AB_2734651 (BioLegend Cat. No. 853502)\u003cbr\u003e\nStructure: FBXO7 is a 522 amino acid protein with a molecular mass of ~60 kD.\u003cbr\u003e\nDistribution: Tissue distribution: FBXO7 is ubiquitously expressed in the many tissues including brain and muscle. Cellular distribution: Cytoplasmic and nucleus.\u003cbr\u003e\nFunction: FBXO7 is involved in the ubiquitin-proteasome system, and defects in FBXO7 cause Parkinson's disease-like phenotypes.\u003cbr\u003e\nInteraction: FBXO7 interacts with the members of the SCF (SKP1\/cullin‐1\/F‐box protein) complex.\u003cbr\u003e\nBiology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: Vingill S, et al. 2016. EMBO J. 35: 2008-25. Zhao T, et al. 2011. PLoS One. 6:e16983. Zhou ZD, et al. 2015. Hum. Mol. Genet. 24:6314-30.\u003cbr\u003e\nGene ID: 25793\u003cbr\u003e\nUniProt: View information about FBXO7 on UniProt.org\u003cbr\u003e\nClone: W16207A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: F-box only protein 7\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866308989097,"sku":"853501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-853501","provider":"Iright","version":"1.0","type":"link"}