{"product_id":"biolegend-870201","title":"Biolegend, 870201, Purified anti-UBE2O Antibody, 25μg","description":"\u003cp\u003eUbiquitin-conjugating enzyme E2 O (UBE2O) is an E2\/E3 hybrid enzyme that displays both E2 and E3 ligases activities and mediates ubiquitination of many target proteins involved in a wide range of processes, including the 5’-AMP-activated protein kinase catalytic subunit a2 (AMPKa2), tumor suppressor ubiquitin carboxyl-terminal hydrolase BAP1, mixed-lineage leukemia (MLL) protein, SMAD family member 6 (SMAD6), transcription factor c-Maf, and free ribosomal proteins. Abnormal expression and mutations in UBE2O are observed in tumorigenesis and are associated with poor prognosis of gastric, lung, breast, and prostate cancers.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human UBE2O protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 5.0 µg\/mL. For immunocytochemistry, a concentration range of 0.5 - 10 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2820188 (BioLegend Cat. No. 870201) AB_2820189 (BioLegend Cat. No. 870202)\u003cbr\u003e\nStructure: Human UBE2O is a 1,292 amino acid protein with a predicted molecular mass of 141 kD and observed molecular mass of ~200 kD\u003cbr\u003e\nDistribution: Tissue distribution: Brain, skeletal muscle, heart Cellular distribution: Nucleus, cytosol\u003cbr\u003e\nFunction: UBE2O is an E2\/E3 hybrid ubiquitin-protein ligase that is crucial for protein ubiquitination and degradation.\u003cbr\u003e\nCell Type: Astrocytes, Microglia, Neurons, Oligodendrocytes\u003cbr\u003e\nBiology Area: Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Ubiquitin\/Protein Degradation\u003cbr\u003e\nMolecular Family: Ligases\u003cbr\u003e\nAntigen References: Ullah K, et al. 2019. FEBS J. 286(11):2018-2034. Chen S, et al. 2018. J Biol Chem. 293(29):11296-11309. Mashtalir N, et al. 2014. Mol Cell. 54(3):392-406.\u003cbr\u003e\nGene ID: 63893\u003cbr\u003e\nUniProt: View information about UBE2O on UniProt.org\u003cbr\u003e\nClone: A18140A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: (E3-independent) E2 ubiquitin-conjugating enzyme, E2\/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kD, Ubiquitin-protein ligase O\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866062377129,"sku":"870201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-870201","provider":"Iright","version":"1.0","type":"link"}