{"product_id":"biolegend-906103","title":"Biolegend, 906103, Biotin anti-6-His Epitope Tag Antibody (Previously Covance catalog# BIOT-156L), 100microl","description":"\u003cp\u003eHis tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Previous lots (B435499 and prior) may have been formulated in Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol. For further information please contact BioLegend Technical Support or Customer Service.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 1 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL (1:1000-1:2000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg\/mL (1:1000-1:2000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Deinert K, et al. 2001. J Biol Chem. 276:6000. (WB) Kojima H, et al. 2001. J Biol Chem. 276:19238. (IF) PubMed Lapinski PE, et al. 2000. J Biol Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (IF)\u003cbr\u003e\nProduct Citations: Yang LF, et al. 2022. Anal Chem. 94:7278. PubMed Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed\u003cbr\u003e\nRRID: AB_2565057 (BioLegend Cat. No. 906103)\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about 6-His on UniProt.org\u003cbr\u003e\nClone: 6-His\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)\u003cbr\u003e\nPreviously: Covance Catalog# BIOT-156L\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862465073321,"sku":"906103","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-906103","provider":"Iright","version":"1.0","type":"link"}