{"product_id":"biolegend-927301","title":"Biolegend, 927301, Avidin\/Biotin Blocking System (Previously Covance catalog# SIG-31126), VO15","description":"\u003cp\u003eVO15\u003cbr\u003e\nFormulation: Blocking Kit. The Avidin and Biotin Solutions contain 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.\u003cbr\u003e\nStorage \u0026amp; Handling: Store between 2°C and 8°C.\u003cbr\u003e\nApplication: IHC, WB\u003cbr\u003e\nRecommended Usage: The optimal working dilution should be determined for each specific assay condition. Blocking Procedure: For paraffin-embedded tissues: To be performed after the antigen retrieval and serum block but before primary antibody incubation. 1. Incubate the Avidin Solution for 10-15 minutes at room temperature. 2. Incubate with the Biotin Solution for 10-15 minutes at room temperature.\u003cbr\u003e\nApplication Notes: BioLegend's Avidin\/Biotin Blocking System is intended for use in immunohistochemistry and immunoblotting procedures. The purpose is to decrease the signal caused by endogenous avidin, biotin, or biotin-binding proteins when immunostaining specimens with detection systems that rely on avidin-biotin or steptavidin-biotin chemistry. Nonspecific staining due to these factors commonly resembles a diffuse, weak, cytoplasmic pattern.\u003cbr\u003e\nProduct Citations: Bednarski E, et al. 2022. MBio. 13:e0084022. PubMed Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed Deisher A, et al. 2022. STAR Protoc. 3:101663. PubMed Feldt J, et al. 2020. Osteoarthr Cartil Open. 2:100124. PubMed Olde Hanhof CJA, et al. 2023. Front Cell Dev Biol. 11:1086823. PubMed Mooney JP, et al. 2022. Wellcome Open Res. 7:134. PubMed Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed Risom T, et al. 2022. Cell. 185:299. PubMed Scur M, et al. 2022. Nat Commun. 13:7272. PubMed Canedo EC, et al. 2021. JCI Insight. 6:. PubMed Elhanani O, et al. 2021. Methods Mol Biol. 2386:147. PubMed\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Avidin\/Biotin Blocking Kit\u003cbr\u003e\nPreviously: Covance Catalog# SIG-31126\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864272457897,"sku":"927301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-927301","provider":"Iright","version":"1.0","type":"link"}