{"product_id":"biolegend-933104","title":"Biolegend, 933104, PE anti-Lck Phospho (Tyr394) Antibody, 100tests","description":"\u003cp\u003eThe Src family tyrosine kinase p56 Lck (Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR\/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR\/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human Src phosphorylated at tyrosine 419\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This clone may recognize other Src family members. This clone was developed using a synthetic peptide corresponding to human Src phosphorylated at tyrosine 419, which displays high sequence homology with the region surrounding Lck tyrosine 394. To confirm specificity to Lck Phospho (Tyr394), the cell line J.Cam1.6 was used. This cell line is a clonal derivative of Jurkat cells that harbors a truncated LCK allele with defective protein expression (Straus, et al. 1992. Cell. 70:585).\u003cbr\u003e\nProduct Citations: Reed JC, et al. 2020. PLoS One. 15:e0236921. PubMed\u003cbr\u003e\nRRID: AB_2820203 (BioLegend Cat. No. 933103) AB_2820204 (BioLegend Cat. No. 933104)\u003cbr\u003e\nStructure: Lck is a 509 amino acid protein with a predicted molecular weight of 58 kD\u003cbr\u003e\nDistribution: Plasma-membrane associated\/T cells\u003cbr\u003e\nFunction: Protein Tyrosine Kinase\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736. Moogk D, et al. 2016. J Immunol. 197:644. Klammt C, et al. 2015. Nat Immunol. 16:961. Cancer Genome Atlas Network. 2015. Cell. 161:1681. Casas J, et al. 2014. Nat Commun. 5:5624 McNeill L, et al. 2007. Immunnity. 27:425. Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40. Kabouridis PS. et al. 2003. Biochem J. 371:907. Abraham N, et al. 1990. Mol. Cell. Biol. 10:5197.\u003cbr\u003e\nGene ID: 3932\u003cbr\u003e\nUniProt: View information about Lck Phospho Tyr394 on UniProt.org\u003cbr\u003e\nClone: A18002D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lsk, p56lck, LCK Proto-Oncogene, Src Family Tyrosine Kinase, Tyrosine-protein kinase LCK, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, T cell-specific protein-tyrosine kinase\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866058543273,"sku":"933104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-933104","provider":"Iright","version":"1.0","type":"link"}