{"product_id":"biolegend-934802","title":"Biolegend, 934802, Purified anti-IL-18 Antibody, 100μg","description":"\u003cp\u003eInterleukin 18 (IL-18) is a proinflammatory cytokine that participates in both innate and acquired immunity. IL-18 is a part of the IL-1 superfamily that activates T-helper 1 (Th1) and natural killer (NK) cells by inducing IFN-γ production. IL-18 is produced in its inactive precursor form, pro-IL-18 (24 kD), with no signal peptide, accumulating in the cytoplasm. Pro-IL-18 is then processed intracellularly by CASP1 into the mature active form of IL-18 (18 kD) which is secreted extracellularly. Mature IL-18 binds to an inducible receptor, IL-18Rα, and then recruits IL-18Rβ which initiates an immune reaction through TLR\/IL-1R-like signaling by recruiting MyD88 and the NF-κB pathway. IL-18 is associated with Adult-onset Still's disease and pleural tuberculosis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human IL-18 protein of approximately 60 amino acids\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone recognizes human IL-18. For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg\/mL.\u003cbr\u003e\nRRID: AB_2820219 (BioLegend Cat. No. 934801) AB_2820220 (BioLegend Cat. No. 934802)\u003cbr\u003e\nStructure: IL-18 is a 193 amino acid protein with a predicted molecular weight of 22 kD.\u003cbr\u003e\nDistribution: IL-18 is secreted, also localized to the nucleus and cytosol.\u003cbr\u003e\nFunction: Proinflammatory cytokine\u003cbr\u003e\nLigand\/Receptor: Binds inducible receptor IL-18Rα, and then recruits IL-18Rβ\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Adachi O, et al. 1998. Cell Press. 9:1:143. Akira S, et al. 2000. Curr. Op. Imm. 12:1:59. Van de Veerdonk, et al. 2011. Trends in Imm. 32:3:110. Canna S, et al. 2017. Jour. of All. and Clin. Imm. 139:5:1698. Muhl H, et al. 2019. Cell Sig. 63:109388.\u003cbr\u003e\nGene ID: 3606\u003cbr\u003e\nUniProt: View information about IL-18 on UniProt.org\u003cbr\u003e\nClone: W18160A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL18, IL-18, Interleukin 18, Interleukin-18, IL-1 Gamma, Interleukin-1 Gamma, IFN-Gamma-Inducing Factor\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866334187689,"sku":"934802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-934802","provider":"Iright","version":"1.0","type":"link"}