{"product_id":"biolegend-934901","title":"Biolegend, 934901, Purified anti-EED Antibody, 25μg","description":"\u003cp\u003eEmbryonic ectoderm development (EED) is a member of the polycomb-group (PcG) family. The PcG family members form multimeric protein complexes called polycomb repressive complexes (PRCs). These polycomb repressive complexes (PRCs) epigenetically silence gene expression and are involved in a variety of biological processes such as cell cycle control, stem cell plasticity, cellular senescence and cancer.  There are multiple isoforms (~4), which seem to interact differently with different PRCs. Isoform 1 of EED also has an alternate phosphorylated form. Diseases associated with EED include Cohen-Gibson syndrome and Weaver syndrome.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human EED protein of approximately 250 amino acids\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedIP, ICC, KO\/KD-WB - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg\/test. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone recognizes 3 isoforms of human and mouse EED.For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg\/mL.\u003cbr\u003e\nRRID: AB_2820221 (BioLegend Cat. No. 934901) AB_2820222 (BioLegend Cat. No. 934902)\u003cbr\u003e\nStructure: EED is an ~440 amino acid protein with 4 isoforms of a predicted molecular weight of around 50 kD.\u003cbr\u003e\nDistribution: EED mainly localizes to the nucleoplasm.\u003cbr\u003e\nFunction: Maintains transcriptionally repressive state of genes.\u003cbr\u003e\nInteraction: EZH2\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Cell Cycle\/DNA Replication, Cell Death, Chromatin Remodeling\/Epigenetics, Stem Cells\u003cbr\u003e\nAntigen References: Van der Vlag J, et al. 1999. Nat. Gen. 23:474. Martin C, et al. 2006. J. Bol. 281:13:8365. Cao Q, et al. 2014. Nat. Comm. 5:3127. Kuzmichev A, et al. 2004. Mol. Cell. 14:183. Vire E, et al. 2005. Nat. 439:871. Schuettengruber B, et al. 2009. Development. 136: 353.\u003cbr\u003e\nGene ID: 8726\u003cbr\u003e\nUniProt: View information about EED on UniProt.org\u003cbr\u003e\nClone: W18151A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Embryonic Ectoderm Development, Embryonic Ectoderm Development Protein, Polycomb Protein EED\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866334089385,"sku":"934901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-934901","provider":"Iright","version":"1.0","type":"link"}