{"product_id":"biolegend-936601","title":"Biolegend, 936601, Purified anti-MUT Antibody, 25μg","description":"\u003cp\u003eMUT encodes MCM (Methylmalonyl-CoA mutase), which resides in the mitochondria to metabolize branched and odd-chain fatty acids. Utilizing cobalamin as a cofactor, MCM also isomerizes methylmalonyl-CoA to make succinyl-CoA, an important intermediate in the Kreb’s Cycle. Mutations in the gene can lead to disruptions in amino acid metabolism and may alter lipid and glucose homeostasis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human MUT protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: When tested for ICC on 4% PFA-fixed HeLa cells, this clone produced punctate nuclear staining, which is inconsistent with the reported mitochondrial matrix localization of MUT. We therefore do not recommend this clone for the application.\u003cbr\u003e\nRRID: AB_2888896 (BioLegend Cat. No. 936601) AB_2888896 (BioLegend Cat. No. 936602)\u003cbr\u003e\nStructure: MUT is a 750 amino acid protein with a predicted molecular weight of 83 kD.\u003cbr\u003e\nDistribution: MUT expression is localized to the cytosol and the mitochondria.\u003cbr\u003e\nFunction: Fatty-acid metabolism; isomerase\u003cbr\u003e\nInteraction: Adenosylcobalamin (Adocbl)\u003cbr\u003e\nAntigen References: 1. Janata J, et al. 1997. Hum. Mol. Gen. 6(9):1457-64. 2. Worgan LC, et al. 2006. Hum. Mutat. 27(1):31-43. 3. Mancia F, et al. 1998. Cell. 6(6):711-20. 4. Reid M, et al. 2017. Cell. 171(4):736-37. 5. Schneller J, et al. 2015. Mol. Ther. 23(1):70.\u003cbr\u003e\nGene ID: 4594\u003cbr\u003e\nUniProt: View information about MUT on UniProt.org\u003cbr\u003e\nClone: W18259A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Methylmalonyl-CoA Mutase, Methylmalonyl Coenzyme A Mutase, MCM, Truncated Methylmalonyl-CoA Mutase Variant, MMUT, EC 5.4.99.2, Methylmalonyl-CoA Isomerase\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866337759401,"sku":"936601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-936601","provider":"Iright","version":"1.0","type":"link"}