{"product_id":"biolegend-940001","title":"Biolegend, 940001, Purified anti-Fumarase Antibody, 25μg","description":"\u003cp\u003eFumarate hydratase, or fumarase, is a Krebs cycle enzyme that catalyzes the conversion of fumarate to L-malate. A cytosolic isoform of Fumarase has been described that is required for arginine metabolism and the urea cycle. Inactivating germline mutations of the FH gene are found in patients with hereditary leiomyomatosis and renal cell cancer syndrome, which predisposes patients to increased risk of developing uterine and cutaneous leipmyomas and renal cell carcinomas. Mechanistically, elevated fumarate functions as an oncometabolite by inhibiting the activity of α-ketoglutarate dependent enzymes, such as hypoxia-inducible factor prolyl hydroxylases (PHDs) and ten-eleven translocation (TET) dioxygenases.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human fumarase protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was tested for ICC on HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods were compatible fumarase staining. This clone failed to immunoprecipitate fumarase from HeLa extracts. We therefore do not recommend this clone for the application.\u003cbr\u003e\nRRID: AB_2890856 (BioLegend Cat. No. 940001) AB_2890856 (BioLegend Cat. No. 940002)\u003cbr\u003e\nStructure: Fumarase is a 510 amino acid protein with a predicted molecular weight of 55 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Mitochondrial, Cytosolic\u003cbr\u003e\nInteraction: H2AZ1\u003cbr\u003e\nBiology Area: Cell Biology, Mitochondrial Function\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: Laukka T, et al. 2016. J Biol Chem. 291:4256-65. Sciavoceli M, et al. 2016. Nature. 537:544-547.\u003cbr\u003e\nGene ID: 2271\u003cbr\u003e\nUniProt: View information about Fumarase on UniProt.org\u003cbr\u003e\nClone: W18319C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fumarate Hydratase, FH\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866339004585,"sku":"940001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-940001","provider":"Iright","version":"1.0","type":"link"}