{"product_id":"biolegend-941301","title":"Biolegend, 941301, Purified anti-TIF1β (KAP-1, TRIM28) Antibody, 25μg","description":"\u003cp\u003eTIFβ (transcription intermediary factor 1-beta) is an 89 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full-length recombinant mouse TIF1β protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: - BioLegend offers two monoclonal antibodies, clones 20A1 and W19027A that bind TIF1β (KAP-1, TRIM28). 20A1 is a mouse IgG1, κ isotype; W19027A is a rat IgG2a, κ isotype Both clones detect human and mouse TIF1β by western blot; 20A1 binds TIF1β with a higher affinity than W19027A for this application, but also shows a higher background Both clones are validated for immunocytochemistry on human cells W19027A is validated for immunoprecipitation, 20A1 has not been tested for this application - Clone W19027A was tested for immunocytochemistry using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. Both methods were compatible with TIF1β staining.\u003cbr\u003e\nRRID: AB_2888901 (BioLegend Cat. No. 941301) AB_2888901 (BioLegend Cat. No. 941302)\u003cbr\u003e\nStructure: TIF1β is an 835 amino acid protein with a predicted molecular weight of 89 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Nucleus\u003cbr\u003e\nFunction: Chromatin remodeling\u003cbr\u003e\nAntigen References: 1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366. 2. Schultz DC, et al. 2002. Genes Dev. 16:919. 3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859. 4. Friedman JR, et al. 1996. Genes Dev. 10:2067. 5. Messerschmidt DM, et al. 2012. Science 335:1499. 6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.\u003cbr\u003e\nGene ID: 10155\u003cbr\u003e\nUniProt: View information about TIF1beta on UniProt.org\u003cbr\u003e\nClone: W19027A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tripartite Motif Containing 28; RING Finger Protein 96; Transcription Intermediary Factor1-β, KRAB [Kruppel-Associated Box Domain]-Associated Protein 1; KRAB-Associated Protein 1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866341986473,"sku":"941301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-941301","provider":"Iright","version":"1.0","type":"link"}