{"product_id":"biolegend-946406","title":"Biolegend, 946406, PE anti-γ-Catenin Antibody, 100tests","description":"\u003cp\u003eγ-catenin, also referred to as JUP and plakoglobin, is a member of the Armadillo family of proteins that are required for Wnt signaling. γ-catenin was originally described as a structural component of desmosomes and adherens junctions, where it links E-cadherins to the actin cytoskeleton. The presence of this complex is critical for cell migration and adhesion. While γ-catenin shows high overall similarity to the homolologous β-catenin, notably in the armadillo domain rich central region, γ-catenin has distinct N- and C-terminal regions, which allows interaction with protein partners functionally distinct with those of other catenins. Additionally, γ-catenin shows tumor suppressor activity by downregulation of oncogenic Wnt signaling. This negative regulation occurs through multiple mechanisms: a. displacement of β-catenin from junctional complexes thereby promoting its ubiquitin-dependent degradation, b. disrupting formation of the TCF- β-catenin transcriptional activator complex by directly binding β-catenin, c. stimulating the nuclear export of the SOX4 transcription factor. γ-catenin also plays a role in maintaining embryonic stem cells in a pluripotent state.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full-length recombinant chicken γ-catenin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This clone was tested for ICC on MCF7 (positive control) and MOLT-4 (negative control) cells using multiple fixation and permeabilization conditions. A brief summary of these results is listed below: methanol only: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Moderate nuclear background staining observed in MCF7 cells. 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells. 4% PFA plus methanol: 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.\u003cbr\u003e\nRRID: AB_2927950 (BioLegend Cat. No. 946405) AB_2927950 (BioLegend Cat. No. 946406)\u003cbr\u003e\nStructure: γ-catenin is a 745 amino acid protein with a predicted molecular weight of 82 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Cytoskeleton and plasma membrane associated\u003cbr\u003e\nFunction: Cell to cell adhesion, cell signaling\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Signal Transduction\u003cbr\u003e\nAntigen References: Zhurinksy J, et al. 2000. J Cell Sci. 18:3127. Mahendram S, et al. 2013. PLoS ONE. 8:e65320. Aktary Z, et al. 2017. Oncotarget. 8:32270.\u003cbr\u003e\nGene ID: 3728\u003cbr\u003e\nUniProt: View information about gamma-Catenin on UniProt.org\u003cbr\u003e\nClone: 15F11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Junction Plakoglobin, DP3, Catenin (Cadherin-associated Protein), Gamma 80kDa, Desmoplakin III, CTNNG, PDGB, PKGB, Catenin Gamma\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860813271209,"sku":"946406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-946406","provider":"Iright","version":"1.0","type":"link"}