{"product_id":"biolegend-947402","title":"Biolegend, 947402, Purified anti-human cdc2 Phospho (Thr14) Antibody, 100microl","description":"\u003cp\u003eCyclin-dependent kinase 1 (Cdk1), also known as cell division control protein 2 (cdc2), is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, cdc2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-cdc2 complex is activated during G2 and early mitosis by cdc25-mediated dephosphorylation. During mitosis, cdc2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. cdc2 can function as a substitute for other cdc family members and drive the progression of the cell cycle in the event of their loss. cdc2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14\u003cbr\u003e\nFormulation: Phosphate buffer, 0.5% BSA, 0.09% NaN3\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.25 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:4000. For immunocytochemistry, a dilution of 1:50 is recommended. For intracellular flow cytometric staining, the suggested dilution of this reagent is 1:100 per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue. This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.\u003cbr\u003e\nRRID: AB_2904458 (BioLegend Cat. No. 947401) AB_2904458 (BioLegend Cat. No. 947402)\u003cbr\u003e\nStructure: cdc2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/ Mitochondria, nucleus, cytoskeleton, cytoplasm\u003cbr\u003e\nFunction: Serine\/threonine-protein kinase\/ Cell Cycle\u003cbr\u003e\nInteraction: Cyclin B1, Cyclin A, PKMYT1 (MYT1)\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Chow J, et al. 2011. Mol. Cell Biol. 31:1478-91. Diril M, et al. 2012. PNAS. 109:3826-31. Haneke K, et al. 2020. J. Cell. Biol. 219: e201906147. Liao H, et al. 2017. Oncotarget. 8:90662-73. Prevo R, et al. 2018. Cell Cycle. 17:1513-23.\u003cbr\u003e\nGene ID: 983\u003cbr\u003e\nUniProt: View information about cdc2 on UniProt.org\u003cbr\u003e\nClone: A20004B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866067554473,"sku":"947402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-947402","provider":"Iright","version":"1.0","type":"link"}