{"product_id":"cst-12733s","title":"CST,  12733S, Galectin-3\/LGALS3 Antibody","description":"Polyclonal Antibody for studying galectin-3. Validated for Western Blotting. Highly specific and rigorously validated in-house, Galectin-3\/LGALS3 Antibody (CST #12733) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGalectin-3\/LGALS3 Antibody recognizes endogenous levels of total galectin-3\/LGALS3 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human galectin-3\/LGALS3 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nGalectins are a family of β-galactose binding proteins that are characterized by an affinity for poly-N-acetyllactosamine-enriched glycoconjugates and a carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions, including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7). Galectin-3\/LGALS3 is involved in several diverse biological functions. Galectin-3\/LGALS3 binds IgE (8). Galectin-3\/LGALS3 is an unusual protein in that it can be found both extracellularly and intracellularly. Intracellularly, Galectin-3\/LGALS3 can localize to the cytoplasm, nucleus, or both, depending on cell type and experimental conditions. Nuclear Galectin-3\/LGALS3 has been identified as a pre-mRNA splicing factor (9). Galectin-3\/LGALS3 production has been shown to increase during inflammation and in obesity, and the protein itself can have an inflammatory effect under certain conditions (10). Galectin-3\/LGALS3 forms a complex with α3, β1 integrin to act as a surface receptor on endothelial cells for the NG2 proteoglycan, which triggers cell motility and angiogenesis (11). In addition to these functions, Galectin-3\/LGALS3 is also a required factor for the terminal differentiation of epithelial cells (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP35; epididymis secretory sperm binding protein; Gal-3; GAL3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; galectin 3; Galectin-3; GALIG; IgE-binding protein; L-31; L31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LEG3; LGALS2; LGALS3; Mac-2 antigen; MAC2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797578305705,"sku":"12733S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/cst-12733s","provider":"Iright","version":"1.0","type":"link"}