CST, 12778S, NCK1 (5B7) Mouse Monoclonal Antibody

Monoclonal Antibody for studying NCK1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NCK1 (5B7) Mouse Monoclonal Antibody (CST #12778) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NCK1 (5B7) Mouse Monoclonal Antibody recognizes endogenous levels of total NCK1 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to a fragment of human NCK1 protein. Background NCK adapter protein 1 (NCK1), also known as NCK or NCKα, is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2, also known as NCKβ, a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7). Alternate Names Cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; NCK1; NCKalpha; non-catalytic region of tyrosine kinase; SH2/SH3 adaptor protein NCK-alpha Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Mouse IgG1