{"product_id":"cst-31433t","title":"CST,  31433T, Phospho-Rb (Thr821) (E6J1L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Rb (Thr821) phosphate. Validated for WB,WB,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Rb (Thr821) (E6J1L) Rabbit Monoclonal Antibody (CST #31433) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunofluorescence (Immunocytochemistry): 1:12800 - 1:25600\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 67545 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Rb (Thr821) (E6J1L) Rabbit Monoclonal Antibody recognizes endogenous levels of Rb protein only when phosphorylated at Thr821. Reactivity for immunofluorescence is human only.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr821 of human Rb protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4\/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK\/cyclin complexes has been observed (6-8) and cyclin D1 is required for Ser780 phosphorylation (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nexon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB1; retinoblastoma 1; retinoblastoma suspectibility protein; Retinoblastoma-associated protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798073462953,"sku":"31433T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/cst-31433t","provider":"Iright","version":"1.0","type":"link"}