{"product_id":"cst-47012t","title":"CST,  47012T, ROCK2 (E5T5P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ROCK2. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, ROCK2 (E5T5P) Rabbit Monoclonal Antibody (CST #47012) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:200\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nROCK2 (E5T5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ROCK2 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1383 of human ROCK2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nROCK (Rho-associated kinase), a family of serine\/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). ROCK1 is cleaved by caspase-3 at a conserved DETD1113\/G sequence resulting in loss of its C-terminal inhibitory domain (3). ROCK2 is directly cleaved by granzyme B (grB). Cleavage activates ROCK and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase (4). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (5-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nKIAA0619; p164 ROCK-2; Rho associated coiled-coil containing protein kinase 2; Rho kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; ROCK2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 160\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799537242281,"sku":"47012T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/cst-47012t","provider":"Iright","version":"1.0","type":"link"}