{"product_id":"proteintech-10084-2-ap","title":"Proteintech, 10084-2-AP, CD71 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CD71 (10084-2-AP) by Proteintech is a Polyclonal antibody targeting CD71 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n10084-2-AP targets CD71 in WB, IHC, IF\/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-1080 cells, LoVo cells,  HeLa cells,  Jurkat cells,  K-562 cells,  human placenta tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: K-562 cells, MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, monkey, chicken, bovine, duck\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0123 Product name: Recombinant human CD71 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 513-757 aa of BC001188 Sequence: VKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDID Predict reactive species\nFull Name: transferrin receptor (p90, CD71)\nCalculated Molecular Weight: 85 kDa\nObserved Molecular Weight: 90 kDa\nGenBank Accession Number: BC001188\nGene Symbol: CD71\nGene ID (NCBI): 7037\nENSEMBL Gene ID: ENSG00000072274\nRRID: AB_2240403\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P02786\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868825702569,"sku":"10084-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10084-2-ap","provider":"Iright","version":"1.0","type":"link"}