{"product_id":"proteintech-10109-2-ap","title":"Proteintech, 10109-2-AP, DAB2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe DAB2 (10109-2-AP) by Proteintech is a Polyclonal antibody targeting DAB2 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n10109-2-AP targets DAB2 in WB, IHC, IF\/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  RAW 264.7 cells,  rat testis tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nDAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta).  Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13.  The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt\/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, chicken\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0162 Product name: Recombinant human DAB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 340-580 aa of BC003064 Sequence: VDYFGQQFDQISNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVGSPPKGLSIQNGVKQDLESSVQSSPHDSIAIIPPPQSTKPGRGRRTAKSSANDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTSAPAPVGPLVGLGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSPAVSGWNQPSPFAASTPPPVPVVWGPSASVA Predict reactive species\nFull Name: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)\nCalculated Molecular Weight: 82 kDa\nObserved Molecular Weight: 96 kDa\nGenBank Accession Number: BC003064\nGene Symbol: DAB2\nGene ID (NCBI): 1601\nRRID: AB_2089700\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P98082\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868940128425,"sku":"10109-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10109-2-ap","provider":"Iright","version":"1.0","type":"link"}