{"product_id":"proteintech-10206-1-ap","title":"Proteintech, 10206-1-AP, NCK2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NCK2 (10206-1-AP) by Proteintech is a Polyclonal antibody targeting NCK2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n10206-1-AP targets NCK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: U-937 cells\nPositive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nLots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0262 Product name: Recombinant human NCK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 8-235 aa of BC000103 Sequence: IAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWW Predict reactive species\nFull Name: NCK adaptor protein 2\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 43 kDa\nGenBank Accession Number: BC000103\nGene Symbol: NCK2\nGene ID (NCBI): 8440\nRRID: AB_2150571\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43639\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869564850345,"sku":"10206-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10206-1-ap","provider":"Iright","version":"1.0","type":"link"}