{"product_id":"proteintech-10228-1-ap","title":"Proteintech, 10228-1-AP, Nucleobindin 1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Nucleobindin 1 (10228-1-AP) by Proteintech is a Polyclonal antibody targeting Nucleobindin 1 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples\n10228-1-AP targets Nucleobindin 1 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: MCF7 cells,  A431 cells,  HeLa cells,  HepG2 cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\nPositive IF\/ICC detected in: HepG2 cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNucleobindin-1 (NUCB1), also known as Calnuc, is a highly conserved, multifunctional protein widely expressed in tissues and cells.It has been proposed to contain both calcium- and DNA-binding motifs, including two EF-hands, a basic amino-acid-rich region and a leucine zipper.Western blotting revealed a band at approximately 63 kDa, corresponding to the expected size of the NUCB1 protein(PMID: 15255074)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: mouse, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0290 Product name: Recombinant human NUCB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 127-286 aa of BC002356 Sequence: VQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDM Predict reactive species\nFull Name: nucleobindin 1\nCalculated Molecular Weight: 55 kDa\nObserved Molecular Weight: 63 kDa\nGenBank Accession Number: BC002356\nGene Symbol: NUCB1\nGene ID (NCBI): 4924\nRRID: AB_2153655\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q02818\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869568094377,"sku":"10228-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10228-1-ap","provider":"Iright","version":"1.0","type":"link"}