{"product_id":"proteintech-10349-1-ap","title":"Proteintech, 10349-1-AP, NCBP1\/CBP80 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NCBP1\/CBP80 (10349-1-AP) by Proteintech is a Polyclonal antibody targeting NCBP1\/CBP80 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples\n10349-1-AP targets NCBP1\/CBP80 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  human brain tissue,  Jurkat cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human lung cancer tissue,  human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of  a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex  may play a role in pre-mRNA recognition and splicing.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0496 Product name: Recombinant human CBP80 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 46-350 aa of BC001450 Sequence: LESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH Predict reactive species\nFull Name: nuclear cap binding protein subunit 1, 80kDa\nCalculated Molecular Weight: 92 kDa\nObserved Molecular Weight: 80 kDa\nGenBank Accession Number: BC001450\nGene Symbol: NCBP1\nGene ID (NCBI): 4686\nRRID: AB_2150127\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q09161\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868907327657,"sku":"10349-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10349-1-ap","provider":"Iright","version":"1.0","type":"link"}