{"product_id":"proteintech-10412-1-ap","title":"Proteintech, 10412-1-AP, RPA2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RPA2 (10412-1-AP) by Proteintech is a Polyclonal antibody targeting RPA2 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10412-1-AP targets RPA2 in WB, IHC, IF\/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  mouse brain tissue,  SH-SY5Y cells\nPositive IP detected in: mouse kidney tissue\nPositive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser\/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0690 Product name: Recombinant human RPA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 80-270 aa of BC001630 Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE Predict reactive species\nFull Name: replication protein A2, 32kDa\nCalculated Molecular Weight: 29 kDa\nObserved Molecular Weight: 32 kDa\nGenBank Accession Number: BC001630\nGene Symbol: RPA2\nGene ID (NCBI): 6118\nRRID: AB_2269665\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P15927\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868829175977,"sku":"10412-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10412-1-ap","provider":"Iright","version":"1.0","type":"link"}