{"product_id":"proteintech-10675-1-ap","title":"Proteintech, 10675-1-AP, PAIP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PAIP1 (10675-1-AP) by Proteintech is a Polyclonal antibody targeting PAIP1 in WB, IF\/ICC, IP, ELISA applications with reactivity to human, monkey samples\n10675-1-AP targets PAIP1 in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, monkey samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, BxPC-3 cells,  COS-7 cells\nPositive IP detected in: HeLa cells\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIn initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, monkey\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1070 Product name: Recombinant human PAIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 102-400 aa of BC005295 Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ Predict reactive species\nFull Name: poly(A) binding protein interacting protein 1\nCalculated Molecular Weight: 70 kDa\nObserved Molecular Weight: 60 kDa, 51 kDa, 40 kDa\nGenBank Accession Number: BC005295\nGene Symbol: PAIP1\nGene ID (NCBI): 10605\nRRID: AB_2160391\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9H074\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868971389097,"sku":"10675-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10675-1-ap","provider":"Iright","version":"1.0","type":"link"}