{"product_id":"proteintech-10712-1-ap","title":"Proteintech, 10712-1-AP, Cytokeratin 19 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cytokeratin 19 (10712-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 19 in WB, IHC, IF\/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse samples\n10712-1-AP targets Cytokeratin 19 in WB, IHC, IF\/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A431 cells, mouse placenta tissue,  mouse brain tissue,  A549 cells,  HepG2 cells,  HT-29 cells,  MCF-7 cells,  T-47D cells,  SK-BR-3 cells\nPositive IHC detected in: human colon tissue, human breast cancer tissue,  human colon cancer tissue,  human lung cancer tissue,  human oesophagus cancer tissue,  human pancreas tissue,  human skin tissue,  human stomach cancer tissue,  human thyroid cancer tissue,  mouse colon tissue,  mouse pancreas tissue,  mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: Retinal organoids, mouse pancreas tissue\nPositive IF\/ICC detected in: HaCaT cells, HeLa cells,  HepG2 cells\nPositive FC (Intra) detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:20000-1:100000\nImmunohistochemistry (IHC): IHC : 1:3000-1:12000\nImmunofluorescence (IF)-P: IF-P : 1:10-1:100\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, pig, canine, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1085 Product name: Recombinant human CK19 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 80-400 aa of BC007628 Sequence: EKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL Predict reactive species\nFull Name: keratin 19\nCalculated Molecular Weight: 40 kDa\nObserved Molecular Weight: 44-50 kDa\nGenBank Accession Number: BC007628\nGene Symbol: Cytokeratin 19\nGene ID (NCBI): 3880\nRRID: AB_2133325\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P08727\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868824588457,"sku":"10712-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10712-1-ap","provider":"Iright","version":"1.0","type":"link"}