{"product_id":"proteintech-10771-1-ap","title":"Proteintech, 10771-1-AP, PACT Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PACT (10771-1-AP) by Proteintech is a Polyclonal antibody targeting PACT in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n10771-1-AP targets PACT in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, MCF-7 cells,  C2C12 cells,  PC-12 cells\nPositive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2\/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1\/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1214 Product name: Recombinant human PRKRA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC009470 Sequence: MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK Predict reactive species\nFull Name: protein kinase, interferon-inducible double stranded RNA dependent activator\nCalculated Molecular Weight: 34 kDa\nObserved Molecular Weight: 31-34 kDa\nGenBank Accession Number: BC009470\nGene Symbol: PRKRA\nGene ID (NCBI): 8575\nRRID: AB_2170496\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O75569\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868907589801,"sku":"10771-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10771-1-ap","provider":"Iright","version":"1.0","type":"link"}