{"product_id":"proteintech-10875-1-ap","title":"Proteintech, 10875-1-AP, HSP47\/SERPINH1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HSP47\/SERPINH1 (10875-1-AP) by Proteintech is a Polyclonal antibody targeting HSP47\/SERPINH1 in WB, IHC, IF\/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n10875-1-AP targets HSP47\/SERPINH1 in WB, IHC, IF\/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-1080 cells, A2780 cells,  A431 cells,  HeLa cells,  NIH\/3T3 cells,  BALB\/3T3 clone A31 cells\nPositive IHC detected in: human stomach cancer tissue, human colon tissue,  human hepatocirrhosis tissue,  human lung cancer tissue,  mouse kidney tissue,  mouse lung tissue,  rat kidney tissue,  rat lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\nPositive FC (Intra) detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47,  which is required for the correct folding and secretion of collagen (PMID: 30128672). SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease,  keloid scars, and lung fibrosis (PMID: 32091407). SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines (PMID: 24595468). SERPINH1 regulates the Wnt\/β-catenin signaling pathway and modulates gastric cancer (GC) progression (PMID: 32091407). SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer (PMID: 32091407). SERPINH1 promotes invasion and metastasis of breast cancer cells by  regulating the expression of several extracellular matrix (ECM) proteins (PMID: 25744716). Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1301 Product name: Recombinant human SERPINH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-418 aa of BC014623 Sequence: MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL Predict reactive species\nFull Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)\nCalculated Molecular Weight: 46 kDa\nObserved Molecular Weight: 46 kDa\nGenBank Accession Number: BC014623\nGene Symbol: HSP47\/SERPINH1\nGene ID (NCBI): 871\nRRID: AB_2301500\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P50454\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868882096297,"sku":"10875-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10875-1-ap","provider":"Iright","version":"1.0","type":"link"}