{"product_id":"proteintech-10985-1-ap","title":"Proteintech, 10985-1-AP, NCLN Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NCLN (10985-1-AP) by Proteintech is a Polyclonal antibody targeting NCLN in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n10985-1-AP targets NCLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse pancreas tissue\nPositive IHC detected in: human stomach cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNicalin, also named as NCLN, is a key component of a protein complex that antagonizes Nodal signaling, which in vertebrates is involved in induction of the mesoderm and endoderm. Nicalin is highest expression in pancreas and skeletal muscle, with somewhat lower expression in heart and very low expression in all other tissues examined. Endogenous nicalin has an apparent molecular mass of 60 kDa. Nicalin was also detected in a 500- to 550-kDa complex containing NOMO2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1430 Product name: Recombinant human NCLN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 214-563 aa of BC013283 Sequence: EDLPTIVIVAHYDAFGVAPWLSLGADSNGSGVSVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDNLDHTDSSLLQDNVAFVLCLDTVGRGSSLHLHVSKPPREGTLQHAFLRELETVAAHQFPEVRFSMVHKRINLAEDVLAWEHERFAIRRLPAFTLSHLESHRDGQRSSIMDVRSRVDSKTLTRNTRIIAEALTRVIYNLTEKGTPPDMPVFTEQMQIQQEQLDSVMDWLTNQPRAAQLVDKDSTFLSTLEHHLSRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAVFDLLLAVGIAAYLGMAYVAVQHFSLLYKTVQRLLVKAKTQ Predict reactive species\nFull Name: nicalin homolog (zebrafish)\nCalculated Molecular Weight: 60 kDa\nObserved Molecular Weight: 60-64 kDa\nGenBank Accession Number: BC013283\nGene Symbol: NCLN\nGene ID (NCBI): 56926\nRRID: AB_2150898\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q969V3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887733592233,"sku":"10985-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10985-1-ap","provider":"Iright","version":"1.0","type":"link"}