{"product_id":"proteintech-10991-1-ap","title":"Proteintech, 10991-1-AP, FHL1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FHL1 (10991-1-AP) by Proteintech is a Polyclonal antibody targeting FHL1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat, zebrafish samples\n10991-1-AP targets FHL1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse skeletal muscle tissue, HEK-293 cells,  human brain tissue,  mouse colon tissue,  rat skeletal muscle tissue\nPositive IP detected in: mouse skeletal muscle tissue\nPositive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFour-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, zebrafish\nCited Reactivity: human, mouse, rat, zebrafish\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1448 Product name: Recombinant human FHL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC010998 Sequence: MAEKFDCHYCRDPLQGKKYVQKDGHHCCLKCFDKFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGSFFPKGEDFYCVTCHETKFAKHCVKCNKAITSGGITYQDQPWHADCFVCVTCSKKLAGQRFTAVEDQYYCVDCYKNFVAKKCAGCKNPITGFGKGSSVVAYEGQSWHDYCFHCKKCSVNLANKRFVFHQEQVYCPDCAKKL Predict reactive species\nFull Name: four and a half LIM domains 1\nCalculated Molecular Weight: 32 kDa\nObserved Molecular Weight: 32 kDa\nGenBank Accession Number: BC010998\nGene Symbol: FHL1\nGene ID (NCBI): 2273\nRRID: AB_2104230\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13642\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868855161001,"sku":"10991-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-10991-1-ap","provider":"Iright","version":"1.0","type":"link"}