{"product_id":"proteintech-11029-1-ap","title":"Proteintech, 11029-1-AP, PSMB4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PSMB4 (11029-1-AP) by Proteintech is a Polyclonal antibody targeting PSMB4 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse samples\n11029-1-AP targets PSMB4 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, mouse skeletal muscle tissue,  human placenta tissue,  human skeletal muscle tissue,  MCF-7 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells,  HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:10000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPSMB4(Proteasome subunit beta type-4) is also named as PROS26 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The human protein PSMB4 is known mainly as an interaction partner for several proteins, suggesting an important function of binding not only to the lid, but also to the 20 S core of the proteasome. PSMB4 might be a major site for proteasome regulation, where signals from the outside might be transduced to the protease activities inside(PMID:15660529 ). The full length 30 kDa protein has a propeptide of 45 amino acid.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, pig, monkey\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1500 Product name: Recombinant human PSMB4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC010088 Sequence: MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQTATVTEKGVEIEGPLSTETNWDIAHMISGFE Predict reactive species\nFull Name: proteasome (prosome, macropain) subunit, beta type, 4\nCalculated Molecular Weight: 29 kDa\nObserved Molecular Weight: 25 kDa\nGenBank Accession Number: BC010088\nGene Symbol: PSMB4\nGene ID (NCBI): 5692\nRRID: AB_2172040\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P28070\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868987216041,"sku":"11029-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-11029-1-ap","provider":"Iright","version":"1.0","type":"link"}