{"product_id":"proteintech-11280-1-ap","title":"Proteintech, 11280-1-AP, EIF4A2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF4A2 (11280-1-AP) by Proteintech is a Polyclonal antibody targeting EIF4A2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n11280-1-AP targets EIF4A2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, mouse ovary tissue,  Raji cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human breast cancer tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1810 Product name: Recombinant human EIF4A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 108-407 aa of BC015842 Sequence: APTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI Predict reactive species\nFull Name: eukaryotic translation initiation factor 4A, isoform 2\nCalculated Molecular Weight: 47 kDa\nObserved Molecular Weight: 47-50 kDa\nGenBank Accession Number: BC015842\nGene Symbol: EIF4A2\nGene ID (NCBI): 1974\nRRID: AB_2097378\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q14240\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868911259817,"sku":"11280-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-11280-1-ap","provider":"Iright","version":"1.0","type":"link"}