{"product_id":"proteintech-11528-1-ap","title":"Proteintech, 11528-1-AP, SRP14 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SRP14 (11528-1-AP) by Proteintech is a Polyclonal antibody targeting SRP14 in WB, IF\/ICC, IP, ELISA applications with reactivity to human, rat samples\n11528-1-AP targets SRP14 in WB, IF\/ICC, IP, ELISA applications and shows reactivity with human, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, HeLa cells,  human heart tissue\nPositive IP detected in: A549 cells\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSignal recognition particle(SRP) acts in three distinct ways: 1 it binds to the signal sequence of the nascent polypeptide to be translocated, which is exposed on the surface of the translating ribosome; 2 it temporarily retards the nascent polypeptide from further elongation; 3 it mediates docking of the SRP-ribosome-nascent polypeptide chain complex to the RER membrane via the heterodimeric SRP-receptor (SR). SRP14, also named as 18 kDa Alu RNA-binding protein, is a 136 amino acid protein. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. SRP14 protein is a component of signal-recognition-particle that has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. SRP14 form a heterodimer with SRP9, which recognizes Alu RNA and the related 7SL RNA.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2099 Product name: Recombinant human SRP14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-136 aa of BC035495 Sequence: MVLLESEQFLTELTRLFQKCRTSGSVYITLKKYDGRTKPIPKKGTVEGFEPADNKCLLRATDGKKKISTVVSSKEVNKFQMAYSNLLRANMDGLKKRDKKNKTKKTKAAAAAAAAAPAAAATAPTTAATTAATAAQ Predict reactive species\nFull Name: signal recognition particle 14kDa (homologous Alu RNA binding protein)\nCalculated Molecular Weight: 136 aa, 15 kDa\nObserved Molecular Weight: 18 kDa\nGenBank Accession Number: BC035495\nGene Symbol: SRP14\nGene ID (NCBI): 6727\nRRID: AB_2194708\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P37108\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868899430569,"sku":"11528-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-11528-1-ap","provider":"Iright","version":"1.0","type":"link"}