{"product_id":"proteintech-11536-1-ap","title":"Proteintech, 11536-1-AP, PREP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PREP (11536-1-AP) by Proteintech is a Polyclonal antibody targeting PREP in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n11536-1-AP targets PREP in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human lung tissue,  Jurkat cells,  mouse lung tissue\nPositive IHC detected in: human ovary tumor tissue, human colon cancer tissue,  human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:200-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, hamster\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2101 Product name: Recombinant human PREP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 360-710 aa of BC030636 Sequence: ILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP Predict reactive species\nFull Name: prolyl endopeptidase\nCalculated Molecular Weight: 710 aa, 81 kDa\nObserved Molecular Weight: 81 kDa\nGenBank Accession Number: BC030636\nGene Symbol: PREP\nGene ID (NCBI): 5550\nRRID: AB_2284249\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P48147\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868972241065,"sku":"11536-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-11536-1-ap","provider":"Iright","version":"1.0","type":"link"}