{"product_id":"proteintech-11582-1-ap","title":"Proteintech, 11582-1-AP, PLSCR1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PLSCR1 (11582-1-AP) by Proteintech is a Polyclonal antibody targeting PLSCR1 in WB, IHC, IF\/ICC, IF-Fro, IP, ELISA applications with reactivity to human, mouse, rat samples\n11582-1-AP targets PLSCR1 in WB, IHC, IF\/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, human skeletal muscle tissue,  mouse skeletal muscle tissue,  mouse lung tissue,  A549 cells,  HepG2 cells,  human placenta tissue\nPositive IP detected in: mouse lung tissue\nPositive IHC detected in: mouse spleen tissue, human kidney tissue,  human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-Fro detected in: rat brain tissue, mouse brain tissue\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:20-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids (\"scrambling\") in a Ca2+ dependent manner,  which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2172 Product name: Recombinant human PLSCR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC021100 Sequence: MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW Predict reactive species\nFull Name: phospholipid scramblase 1\nCalculated Molecular Weight: 318 aa, 35 kDa\nObserved Molecular Weight: 35 kDa\nGenBank Accession Number: BC021100\nGene Symbol: PLSCR1\nGene ID (NCBI): 5359\nRRID: AB_2165659\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O15162\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868891271337,"sku":"11582-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-11582-1-ap","provider":"Iright","version":"1.0","type":"link"}