{"product_id":"proteintech-11865-1-ap","title":"Proteintech, 11865-1-AP, ADAMTS4 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ADAMTS4 (11865-1-AP) by Proteintech is a Polyclonal antibody targeting ADAMTS4 in WB, IHC, ELISA applications with reactivity to human, mouse samples\n11865-1-AP targets ADAMTS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue\nPositive IHC detected in: human liver tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:300-1:600\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS4 is a well-known proteoglycanase and has angiomodulatory properties. The 837 amino acid long human ADAMTS4 protein contains a long signal peptide, a prodomain, a metalloproteinase catalytic domain with zinc-binding motif, a disintegrin-like domain, a central TSR motif, followed by a cysteine-rich region with ten conserved cysteine residues, and a spacer domain. The full-length proform human ADAMTS4 (zymogen form) has been described to be 90 kDa. In extracellular matrix (ECM), ADAMTS4 undergoes further C-terminal cleavage at Lys694-Phe695 and Thr581-Phe582, respectively, to generate two other truncated forms-53 and 40 kDa (PMID:12202483).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse, rat, pig, rabbit\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2443 Product name: Recombinant human ADAMTS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-339 aa of BC030812 Sequence: SQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSARLASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDSGVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALLGVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPLGSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT Predict reactive species\nFull Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4\nCalculated Molecular Weight: 837 aa, 90 kDa\nObserved Molecular Weight: 90 kDa\nGenBank Accession Number: BC030812\nGene Symbol: ADAMTS4\nGene ID (NCBI): 9507\nRRID: AB_2877799\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O75173\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868865384617,"sku":"11865-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-11865-1-ap","provider":"Iright","version":"1.0","type":"link"}