{"product_id":"proteintech-12067-1-ap","title":"Proteintech, 12067-1-AP, ATPIF1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe ATPIF1 (12067-1-AP) by Proteintech is a Polyclonal antibody targeting ATPIF1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n12067-1-AP targets ATPIF1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2400\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe H(+)-ATP synthase is a reversible engine of mitochondria that synthesizes or hydrolyzes ATP upon changes in cell physiology. ATP synthase dysfunction is involved in the onset and progression of diverse human pathologies. ATPIF1 gene encodes mitochondrial ATPase Inhibitory Factor 1 (IF1), also named ATPI, ATPIP or IP. Endogenous IF1 limits ATP depletion when the mitochondrial membrane potential falls below a threshold and the ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Mitochondrial content of IF1 controls the activity of oxidative phosphorylation mediating the shift of cancer cells to an enhanced aerobic glycolysis, thus supporting an oncogenic role of IF1 in cancer.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2704 Product name: Recombinant human ATPIF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC009677 Sequence: MAVTALAARTWLGVWGVRTMQARGFGSDQSENVDRGAGSIREAGGAFGKREQAEEERYFRAQSREQLAALKKHHEEEIVHHKKEIERLQKEIERHKQKIKMLKHDD Predict reactive species\nFull Name: ATPase inhibitory factor 1\nCalculated Molecular Weight: 106 aa, 12 kDa\nObserved Molecular Weight: 12 kDa\nGenBank Accession Number: BC009677\nGene Symbol: ATPIF1\nGene ID (NCBI): 93974\nRRID: AB_2063159\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UII2\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868932067497,"sku":"12067-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12067-1-ap","provider":"Iright","version":"1.0","type":"link"}