{"product_id":"proteintech-12171-1-ap","title":"Proteintech, 12171-1-AP, E2F1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe E2F1 (12171-1-AP) by Proteintech is a Polyclonal antibody targeting E2F1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12171-1-AP targets E2F1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, A431 cells,  HeLa cells,  mouse brain tissue,  NIH\/3T3 cells,  HepG2 cells,  Jurkat cells,  LNCaP cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunohistochemistry (IHC): IHC : 1:50-1:200\n\u003cb\u003eBackground Information\u003c\/b\u003e\nTranscription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1\/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, artemia sinica\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2813 Product name: Recombinant human E2F1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 88-438 aa of BC050369 Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF Predict reactive species\nFull Name: E2F transcription factor 1\nCalculated Molecular Weight: 437 aa, 47 kDa\nObserved Molecular Weight: 55 kDa\nGenBank Accession Number: BC050369\nGene Symbol: E2F1\nGene ID (NCBI): 1869\nRRID: AB_2096958\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q01094\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868840546473,"sku":"12171-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12171-1-ap","provider":"Iright","version":"1.0","type":"link"}