{"product_id":"proteintech-12227-1-ap","title":"Proteintech, 12227-1-AP, EIF4E2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF4E2 (12227-1-AP) by Proteintech is a Polyclonal antibody targeting EIF4E2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12227-1-AP targets EIF4E2 in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells,  MCF-7 cells,  mouse kidney tissue\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2864 Product name: Recombinant human EIF4E2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC021690 Sequence: MNNKFDALKDDDSGDHDQNEENSTQKDGEKEKTERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPGRLGPQRLLFQNLWKPRLNVP Predict reactive species\nFull Name: eukaryotic translation initiation factor 4E family member 2\nCalculated Molecular Weight: 245 aa, 28 kDa\nObserved Molecular Weight: 28 kDa\nGenBank Accession Number: BC021690\nGene Symbol: EIF4E2\nGene ID (NCBI): 9470\nRRID: AB_10642945\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O60573\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868997308585,"sku":"12227-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12227-1-ap","provider":"Iright","version":"1.0","type":"link"}