{"product_id":"proteintech-12387-1-ap","title":"Proteintech, 12387-1-AP, RGMA Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RGMA (12387-1-AP) by Proteintech is a Polyclonal antibody targeting RGMA in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12387-1-AP targets RGMA in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: COLO 320 cells, mouse testis tissue,  HeLa cells,  MOLT-4 cells,  U2OS cells,  Caco-2 cells,  K-562 cells,  mouse brain tissue,  rat brain tissue\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRGMA, also named as RGM, is a member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. It regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. RGMA binding to its receptor NEO1\/neogenin induces activation of RHOA-ROCK1\/Rho-kinase signaling pathway through UNC5B-ARHGEF12\/LARG-PTK2 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1\/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. RGMA is a molecular target for neuroprotection in retinal pathologies(PMID: 20457227). the frequent genetic and epigenetic inactivation of RGMA in CRCs and adenomas along with its in vitro function collectively support its role as a tumor suppressor in colon cells(PMID:19303019 ). There're some different MW in WB detection, 30 kDa mature form, 47-50 kDa isoforms and 60 kDa Glycosylated form.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3058 Product name: Recombinant human RGMA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 136-485 aa of BC015886 Sequence: SAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPGRAAAGLPLAPRPLLGALVPLLALLPVFC Predict reactive species\nFull Name: RGM domain family, member A\nCalculated Molecular Weight: 450 aa, 49 kDa\nObserved Molecular Weight: 49 kDa\nGenBank Accession Number: BC015886\nGene Symbol: RGMA\nGene ID (NCBI): 56963\nRRID: AB_2179366\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96B86\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869493776553,"sku":"12387-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/proteintech-12387-1-ap","provider":"Iright","version":"1.0","type":"link"}